toxreviewТоксикологический вестникToxicological Review0869-79223034-4611Federal Scientific Center of Hygiene named after F.F. Erisman10.47470/0869-7922-2025-33-5-328-336tswsvptoxreview-1022Research ArticleПРОФИЛАКТИЧЕСКАЯ ТОКСИКОЛОГИЯPREVENTIVE TOXICOLOGYВлияние бактериального эндотоксина на уровень цитокинов секретома клеток нейробластомы человека линии SH-SY5YThe effect of bacterial endotoxin on the level of cytokines in the secretome of human neuroblastoma cells of the SH-SY5Y linehttps://orcid.org/0000-0002-7953-7630СкворцовНикита ВладиславовичSkvortsovNikita V.

Научный сотрудник, ФГУП «НИИ гигиены, профпатологии и экологии человека» ФМБА России, 188663, Ленинградская область, г. пос. Кузьмоловский, Россия

e-mail: aelu@yandex.ru

Researcher, Scientific Research Institute of Hygiene, Occupational Pathology and Human Ecology of the Federal Medical Biological Agency of Russia, 188663, Leningrad region, Kuzmolovsky urban settlement, 188663, Russian Federation

e-mail: aelu@yandex.ru

aelu@yandex.ruhttps://orcid.org/0000-0002-7054-6100РоговскаяНадежда ЮрьевнаRogovskayaNadezhda Yu.

Научный сотрудник, ФГУП «НИИ гигиены, профпатологии и экологии человека» ФМБА России, 188663, Ленинградская область, г. пос. Кузьмоловский, Россия

e-mail: nadin-r@mail.ru

Researcher, Scientific Research Institute of Hygiene, Occupational Pathology and Human Ecology of the Federal Medical Biological Agency of Russia, 188663, Leningrad region, Kuzmolovsky urban settlement, 188663, Russian Federation

e-mail: nadin-r@mail.ru

nadin-r@mail.ruhttps://orcid.org/0000-0003-4050-6172БельтюковПетр ПетровичBeltyukovPetr P.

Кандидат мед. наук, ведущий научный сотрудник, ФГУП «НИИ гигиены, профпатологии и экологии человека» ФМБА России, 188663, Ленинградская область, г. пос. Кузьмоловский, Россия

e-mail: beltiukov@gpech.ru

Candidate of Medical Sciences, Leading Researcher, Scientific Research Institute of Hygiene, Occupational Pathology and Human Ecology of the Federal Medical Biological Agency of Russia, Leningrad region, Kuzmolovsky urban settlement, 188663, Russian Federation

e-mail: beltiukov@gpech.ru

beltiukov@gpech.ruБабаковВладимир НиколаевичBabakovVladimir N.

Кандидат биол. наук, заведующий лабораторией № 63, ФГУП «НИИ гигиены, профпатологии и экологии человека» ФМБА России, 188663, Ленинградская область, г. пос. Кузьмоловский, Россия

e-mail: babakov@gpech.ru

Candidate of Biological Sciences, Head of the Laboratory, Scientific Research Institute of Hygiene, Occupational Pathology and Human Ecology of the Federal Medical Biological Agency of Russia, Leningrad region, Kuzmolovsky settlement, 188663, Russian Federation

e-mail: babakov@gpech.ru

babakov@gpech.ruФГУП «Научно-исследовательский институт гигиены, профпатологии и экологии человека» ФМБА РоссииРоссияScientific Research Institute of Hygiene, Occupational Pathology and Human Ecology of the Federal Medical Biological Agensy of RussiaRussian Federation202524112025335328336Copyright © Скворцов Н.В., Роговская Н.Ю., Бельтюков П.П., Бабаков В.Н., 20252025Скворцов Н.В., Роговская Н.Ю., Бельтюков П.П., Бабаков В.Н.Skvortsov N.V., Rogovskaya N.Y., Beltyukov P.P., Babakov V.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.toxreview.ru/jour/article/view/1022Введение

Введение. Проблема выявления бактериальных липополисахаридов (ЛПС) в составе фармацевтических субстанций и реагентов сохраняет свою актуальность. Относительно недавно было показано, что TLR4-PTEN/PI3K/AKT/NF-κB-сигнальный путь принимает участие в нейровоспалении в нейронах гиппокампа крысы после воздействия ЛПС E. coli. Можно сделать вывод, что существует связь между количеством ЛПС и его воздействием на синтез провоспалительных цитокинов / хемокинов и факторов роста в нейронах человека. Для подтверждения теории и поиска изменения уровня цитокинов / хемокинов на уровне белков в нашей работе была выбрана клеточная культура нейробластомы человека линии SH-SY5Y как одна из наиболее изученных нейрональных моделей.

Цель исследования – анализ секретома (цитокинов / хемокинов и факторов роста), участвующего в запуске синтеза цитокинов в нейронах из культуры нейробластомы SH-SY5Y после внесения ЛПС Salmonella typhosa.

Материал и методы

Материал и методы. Для определения содержания цитокинов / хемокинов и факторов роста в клетках культуры нейробластомы SH-SY5Y после воздействия ЛПС использовали многопараметрический иммунофлуоресцентный метод. Потенциальные маркеры определяли методами статистического анализа.

Результаты

Результаты. Воздействие ЛПС из Salmonella typhosa на культуру клеток нейробластомы SH-SY5Y привело к изменениям в секреции цитокинов / хемокинов на уровне белков в период с первого по четвертый день после введения ЛПС.

Ограничения исследования

Ограничения исследования. Исследование in vitro на культуре клеток показало зависимость цитокинового / хемокинового секретома от исследуемых соединений, но результаты нельзя экстраполировать на живой организм.

Заключение

Заключение. Наличие дозозависимых эффектов воздействия бактериальных эндотоксинов на клеточные культуры позволяет рассматривать «определение уровней маркерных цитокинов» в качестве возможного метода для количественного анализа уровня ЛПС.

Соблюдение этических стандартов. Исследование не требует представления заключения комитета по биомедицинской этике или иных документов.

Участие авторов

Участие авторов: Скворцов Н.В. – анализ материала и обработка данных, написание текста; Роговская Н.Ю. – сбор материала, написание текста, статистическая обработка; Бельтюков П.П. – обработка и интерпретация данных, редактирование текста; Бабаков В.Н. – концепция и дизайн исследования, редактирование. Все соавторы – утверждение окончательного варианта статьи, ответственность за целостность всех частей статьи.

Конфликт интересов

Конфликт интересов. Авторы заявляют об отсутствии конфликтов интересов.

Финансирование

Финансирование. Исследование не имело спонсорской поддержки.

Поступила в редакцию

Поступила в редакцию: 21 августа 2025 / Поступила после исправления: 09 сентября 2025 / Принята в печать: 02 октября 2025 / Опубликована: 19 ноября 2025

Introduction

Introduction. The problem of identifying bacterial lipopolysaccharides (LPS) in pharmaceutical substances and reagents remains relevant. recently, it was shown that the TLR4-PTEN/PI3K/AKT/NF-kB signaling pathway participates in neuroinflammation in rat hippocampal neurons after exposure to i LPS. It can be concluded that there is a relationship between the amount of LPS and its effect on the synthesis of pro-inflammatory cytokines, chemokines, and growth factors in human neurons. To confirm the theory and to search for changes in cytokine/chemokines levels at the protein level, we selected the SH-SY5Y human neuroblastoma cell culture as one of the most studied neural models.

The aim of the study was to analyze the secretome (cytokines / chemokines, and growth factors) involved in triggering cytokine synthesis in neurons from the culture of neuroblastoma SH-SY5Y after the action of lipopolysaccharides Salmonella typhosa.

Material and methods

Material and methods. To evaluate the concentrations of cytokines / chemokines, and growth factors in SH-SY5Y neuroblastoma culture cells after exposure to LPS, we utilized a multi-parameter immunofluorescence method. Potential biomarkers were identified through statistical data analysis methods.

Results

Results. The effect of LPS from Salmonella typhosa on neuroblastoma SH-SY5Y cell culture led to changes in cytokine / chemokine secretion at the protein level from the first to the fourth day after LPS administration.

Limitations

Limitations. An in vitro study on cell culture has shown the dependence of the cytokine/chemokine secretome on the studied compounds, but the results cannot be extrapolated to a living organism.

Conclusion

Conclusion. The presence of dose-dependent effects of bacterial endotoxins on cell cultures allows us to consider “determination of marker cytokine levels” as a possible method for quantitative analysis of LPS levels.

Compliance with ethical standards. the study does not require the submission of a biomedical ethics committee opinion or other documents.

Contributions

Contributions: Skvortsov N.V. – collection and processing of material, writing a text; Rogovskaya N.Yu. – collection of material, statistical processing, writing a text; Beltyukov P.P. – data processing and interpretation, text editing; Babakov V.N. – the concept and design of the study, editing. All co-authors are responsible for approving the final version of the article and ensuring the integrity of all parts of the article.

Conflict of interests

Conflict of interests. The authors declare no conflict of interest.

Funding

Funding. The study had no sponsorship.

Received

Received: August 21, 2025 / Revised: September 9, 2025 / Accepted: October 2, 2025 / Published: November 19, 2025

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The authors declare that there are no conflicts of interest present.