toxreviewТоксикологический вестникToxicological Review0869-79223034-4611Federal Scientific Center of Hygiene named after F.F. Erisman10.36946/0869-7922-2018-2-18-27toxreview-114Research ArticleСтатьиСРАВНИТЕЛЬНАЯ И КОМБИНИРОВАННАЯ ТОКСИЧНОСТЬ НАНОЧАСТИЦ ОКСИДОВ АЛЮМИНИЯ, ТИТАНА И КРЕМНИЯ И ЕЁ ОСЛАБЛЕНИЕ КОМПЛЕКСОМ БИОПРОТЕКТОРОВCOMPARATIVE AND COMBINED TOXICITY OF ALUMINIUM, TITANIUM AND SILICON OXIDES NANOPARTICLES AND ITS ALLEVIATION WITH THE COMPLEX OF BIOPROTECTORSМинигалиеваИ. А.MinigalievaI. A.noemail@neicon.ruКацнельсонБ. А.KatsnelsonB. A.noemail@neicon.ruПриваловаЛ. И.PrivalovaL. I.noemail@neicon.ruСутунковаМ. П.SutunkovaM. P.noemail@neicon.ruГурвичВ. Б.GurvichV. B.noemail@neicon.ruШурВ. Я.ShurV. Y.noemail@neicon.ruШишкинаЕ. В.ShishkinaE. V.noemail@neicon.ruВаламинаИ. Е.ValaminaI. E.noemail@neicon.ruМакеевО. Г.MakeyevO. G.noemail@neicon.ruПановВ. Г.PanovV. G.noemail@neicon.ruВараксинА. Н.VaraksinA. N.noemail@neicon.ruКлиноваС. В.KlinovaS. V.noemail@neicon.ruСоловьёваС. В.SolovyevaS. V.noemail@neicon.ruМещеряковаЕ. Ю.MeshtcheryakovaE. Y.noemail@neicon.ruФБУН «Екатеринбургский медицинский - научный центр профилактики и охраны здоровья рабочих промпредприятий» РоспотребнадзораРоссияThe Medical Research Center for Prophylaxis and Health Protection of Industrial Workers, RospotrebnadzorRussian FederationУральский центр коллективного пользования «Современные нанотехнологии», ФГАОУ ВПО «УрФУ имени первого Президента России Б.Н.Ельцина»РоссияUral Center for Shared Use «Modern nanotechnologies», Ural Federal University named after the first President of Russia B.N. YeltsinRussian FederationЦентральная научно-исследовательская лаборатория Уральского государственного медицинского университетаРоссияThe Central Research Laboratory, Ural State Medical UniversityRussian FederationФБУН «Институт промышленной экологии» УрО РАНРоссияInstitute of Industrial Ecology, Ural Branch of the Russian Academy of SciencesRussian Federation201828042018021827Copyright © Минигалиева И.А., Кацнельсон Б.А., Привалова Л.И., Сутункова М.П., Гурвич В.Б., Шур В.Я., Шишкина Е.В., Валамина И.Е., Макеев О.Г., Панов В.Г., Вараксин А.Н., Клинова С.В., Соловьёва С.В., Мещерякова Е.Ю., 20182018Минигалиева И.А., Кацнельсон Б.А., Привалова Л.И., Сутункова М.П., Гурвич В.Б., Шур В.Я., Шишкина Е.В., Валамина И.Е., Макеев О.Г., Панов В.Г., Вараксин А.Н., Клинова С.В., Соловьёва С.В., Мещерякова Е.Ю.Minigalieva I.A., Katsnelson B.A., Privalova L.I., Sutunkova M.P., Gurvich V.B., Shur V.Y., Shishkina E.V., Valamina I.E., Makeyev O.G., Panov V.G., Varaksin A.N., Klinova S.V., Solovyeva S.V., Meshtcheryakova E.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.toxreview.ru/jour/article/view/114

Стабильные суспензии наночастиц (НЧ) оксидов алюминия, титана и кремния, полученные с помощью лазерной абляции соответствующих элементных мишеней 99,99% чистоты под слоем деионизированной воды, вводились крысам внутрибрюшинно 18 раз на протяжении 6 недель изолированно или в различных комбинациях. Развитие субхронической интоксикации оценено большим числом функциональных, биохимических и морфометрических показателей состояния организма. Найдено, что во многих отношениях Al2O3-НЧ наиболее токсичны сами по себе и являются ведущим компонентом изученных комбинаций. Математическое моделирование с помощью построения поверхности отклика (the Response Surface Methodology) показало, что реакция организма на бинарные сочетания исследованных НЧ характеризуется всеми возможными вариантами типов комбинированной токсичности (аддитивность, субаддитивность и супераддитивность однонаправленного действия, различные варианты противонаправленности действия) в зависимости от того, по какому эффекту она оценивается, а также от его уровня и от дозы НЧ. На фоне действия любого третьего вида НЧ тип бинарной комбинированной токсичности остальных двух может существенно измениться. Многие неблагоприятные эффекты действия комбинации [Al2O3-НЧ+TiO2-НЧ+SiO2-НЧ], включая её генотоксичность, были существенно ослаблены пероральным назначением комплекса безвредных биопротекторов.

Stable suspensions of metal/metalloid oxide nanoparticles (MeO-NPs) obtained by laser ablation of 99.99% pure elemental aluminum, titanium or silicon under a layer of deionized water were used separately or in different combinations to induce subchronic intoxications in rats. To this end, MeO-NPs had been repeatedly injected intraperitoneally (i.p.) 18 times during 6 weeks before a large number of functional, biochemical and morphometric indexes for the organism’s status were measured. It was found that, in many respects, the Al2O3-NPs were the most toxic as such and the most dangerous component of the studied combinations. Mathematical modeling with the help of the Response Surface Methodology has shown that the response of the organism to a simultaneous exposure to any two of the MeO-NPs under study is characterized by all possible types of combined toxicity (additivity, subadditivity or superadditivity of unidirectional action and different variants of opposite effects) depending on which outcome this type is estimated for as well as on the levels of the effect and dose. With any third MeO-NP species acting in the background, the type of combined toxicity displayed by the other two can change significantly. Many adverse effects produced by the [Al2O3-NP+TiO2-NP+SiO2-NP]-combination, including its genotoxicity, were substantially attenuated by giving to rats per os during the entire exposure period complex of innocuous bioprotective substances.

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The authors declare that there are no conflicts of interest present.