toxreviewТоксикологический вестникToxicological Review0869-79223034-4611Federal Scientific Center of Hygiene named after F.F. Erisman10.36946/0869-7922-2017-1-47-51toxreview-297Research ArticleСтатьиГЕНДЕРНЫЕ ОСОБЕННОСТИ ИЗМЕНЕНИЯ ЭСТЕРАЗНОГО ПРОФИЛЯ КРОВИ МЫШЕЙ ПРИ ОСТРОМ ОТРАВЛЕНИИ 2-(О-КРЕЗИЛ)-4Н-1,3,2- БЕНЗОДИОКСОФОСФОРИН-2- ОКСИДОМGENDER SPECIFIC CHANGES IN MOUSE BLOOD ESTRERASE PROFILE AT THE ACUTE INTOXICATION BY 2-(О-CRESYL)-4Н-1,3,2-BENZODIOXAPHOSPHORIN-2-OXIDEПрокофьеваД. С.ProkofievaD. S.

кандидат биологических наук, ведущий научный сотрудник лаборатории молекулярной токсикологии и экспериментальной терапии,

188663, Ленинградская обл., Всеволожский район, г.п. Кузьмоловский

188663, Saint Petersburg

darija-p1@yandex.ruШмуракВ. И.ShmurakV. I.

научный сотрудник лаборатории молекулярной токсикологии и экспериментальной терапии,

188663, Ленинградская обл., Всеволожский район, г.п. Кузьмоловский

188663, Saint Petersburg

vladimir.shmurak@gmail.comБодряковаЕ. А.BodryakovaE. A.

старший лаборант лаборатории молекулярной токсикологии и экспериментальной терапии,

188663, Ленинградская обл., Всеволожский район, г.п. Кузьмоловский

188663, Saint Petersburg

bodalena@yandex.ruВойтенкоН. Г.VoitenkoN. G.

кандидат биологических наук, старший научный сотрудник лаборатории молекулярной токсикологии и экспериментальной терапии,

188663, Ленинградская обл., Всеволожский район, г.п. Кузьмоловский 

188663, Saint Petersburg

ngvoitenko@gmail.comФГУП «Научно-исследовательский институт гигиены, профпатологии и экологии человека» ФМБА РоссииРоссияInstitute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency of RussiaRussian Federation201728022017014751Copyright © Прокофьева Д.С., Шмурак В.И., Бодрякова Е.А., Войтенко Н.Г., 20172017Прокофьева Д.С., Шмурак В.И., Бодрякова Е.А., Войтенко Н.Г.Prokofieva D.S., Shmurak V.I., Bodryakova E.A., Voitenko N.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.toxreview.ru/jour/article/view/297

Проведено сравнительное исследование эстеразных профилей крови мышей обоего пола и действия на них различных доз 2-(о-крезил)-4Н-1,3,2-бензодиоксафосфорин-2-оксида (CBDP) спустя 1 час после его подкожного введения животным. Меньшее количество эстераз в крови самцов сделало их более восприимчивыми к действию CBDP по сравнению с самками. Показано, что CBDP в равной степени ингибирует активность карбоксилэстеразы и бутирилхолинэстеразы в сыворотке крови мышей, как самцов, так и самок. Выявлены статистически значимые отличия в степени ингибирования ферментов между самцами и самками, поэтому не рекомендуется использовать смешанные группы животных при проведении исследований ингибиторов сериновых эстераз.

A comparative investigation of the esterase profiles of blood of mice of both genders exposed to various doses of 2-(o-crezyl)-4H-1,3,2-benzodioxaphosphorin-2-oxide (CBDP) was made an hour after its percutaneous injection to animals. A lesser amount of esterase in males blood made them more susceptible to CBDP action as compared to females. It was shown that CBDP equally inhibits the activity of carboxyl esterase and butyryl choline esterase in blood serum of both male and female mice. Statistically significant differences in inhibition degree of enzymes between males and females were found out and therefore it is not recommended to use mixed groups of animals when performing testing of serine esterase inhibitors.

2-(о-крезил)-4Н-132-бензодиоксафосфорин-2-оксидотравлениесериновые эстеразыингибитормышигендерные отличия2-(o-crezyl)-4H-132-benzodioxaphosphorin-2-oxidepoisoningserine esteraseinhibitormicegender differencesReferencesLi B., Sedlacek M., Manoharan I., Boopathy R., Duysen E.G., Masson P., Lockridge O. Butyrylcholinesterase, paraoxonase, and albumin esterase, but not carboxylesterase, are present in human plasma. Biochem. Pharmacol. 2005; 70(11):1673-84.Li B., Sedlacek M., Manoharan I., Boopathy R., Duysen E.G., Masson P., Lockridge O. Butyrylcholinesterase, paraoxonase, and albumin esterase, but not carboxylesterase, are present in human plasma. Biochem. Pharmacol. 2005; 70(11):1673-84.Bahar F.G., Ohura K., Ogihara T., Imai T. Species Difference of Esterase Expression and Hydrolase Activity in Plasma. J. Pharm. Sci. 2012; 101(10):3979-88.Bahar F.G., Ohura K., Ogihara T., Imai T. Species Difference of Esterase Expression and Hydrolase Activity in Plasma. J. Pharm. Sci. 2012; 101(10):3979-88.Berry l.M., Wollenberg l., Zhao Z. Esterase activities in the blood, liver and intestine of several preclinical species and humans. Drug Metab. Lett. 2009; 3(2):70-77.Berry l.M., Wollenberg l., Zhao Z. Esterase activities in the blood, liver and intestine of several preclinical species and humans. Drug Metab. Lett. 2009; 3(2):70-77.Bełtowski J., Wójcicka G., Marciniak A. Species- and substrate-specific stimulation of human plasma paraoxonase 1 (PON1) activity by high chloride concentration. Acta Biochim. Pol. 2002; 49(4):927-936.Bełtowski J., Wójcicka G., Marciniak A. Species- and substrate-specific stimulation of human plasma paraoxonase 1 (PON1) activity by high chloride concentration. Acta Biochim. Pol. 2002; 49(4):927-936.Clement J G., Erhardt N. Serum carboxylesterase activity in various strains of rats: sensitivity to inhibition by CBDP (2-/o-cresyl/4H:1:3:2-benzodioxaphosphorin2-oxide). Arch. Toxicol. 1990; 64(5): 414– 416.Clement J G., Erhardt N. Serum carboxylesterase activity in various strains of rats: sensitivity to inhibition by CBDP (2-/o-cresyl/4H:1:3:2-benzodioxaphosphorin2-oxide). Arch. Toxicol. 1990; 64(5): 414– 416.Duysen E.G., Koentgen F., Williams G.R., timperley c.m., schopfer l.m., cerasoli d.m., lockridge o. Production of ES1 plasma carboxylesterase knockout mice for toxicity studies. Chem. Res. Toxicol. 2011; 24(11): 1891-1898.Duysen E.G., Koentgen F., Williams G.R., timperley c.m., schopfer l.m., cerasoli d.m., lockridge o. Production of ES1 plasma carboxylesterase knockout mice for toxicity studies. Chem. Res. Toxicol. 2011; 24(11): 1891-1898.Garrett T.l., Rapp C.M., Grubbs R.D., Schlager J.J., lucot J.B. A murine model for sarin exposure using the carboxylesterase inhibitor CBDP. Neurotoxicology. 2010; 31(5):502-8.Garrett T.l., Rapp C.M., Grubbs R.D., Schlager J.J., lucot J.B. A murine model for sarin exposure using the carboxylesterase inhibitor CBDP. Neurotoxicology. 2010; 31(5):502-8.Maxwell D.M., Brecht K.M., Lenz D.E., O’Neill B.L. Effect of carboxylesterase inhibition on carbamate protection against soman toxicity. J. Pharmacol. Exp. Ther. 1988; 246(3):986-991.Maxwell D.M., Brecht K.M., Lenz D.E., O’Neill B.L. Effect of carboxylesterase inhibition on carbamate protection against soman toxicity. J. Pharmacol. Exp. Ther. 1988; 246(3):986-991.Clement J.G. Importance of aliesterase as a detoxification mechanism for soman (Pinacolyl methylphosphonofluoridate) in mice. Biochem. Pharmacol. 1984; 33(23):3807-3811.Clement J.G. Importance of aliesterase as a detoxification mechanism for soman (Pinacolyl methylphosphonofluoridate) in mice. Biochem. Pharmacol. 1984; 33(23):3807-3811.Jokanović M. Role of carboxylesterase in soman, sarin and tabun poisoning in rats. Pharmacol. Toxicol. 1989;65(3):181-184.Jokanović M. Role of carboxylesterase in soman, sarin and tabun poisoning in rats. Pharmacol. Toxicol. 1989;65(3):181-184.Yang Z.P.,DettbarnW-D. Prevention of Tolerance to the Organophosphorus Anticholinesterase Paraoxon with Carboxylesterase Inhibitors. Biochemical. Pharmacology. 1998; 55(9): 1419–1426.Yang Z.P.,DettbarnW-D. Prevention of Tolerance to the Organophosphorus Anticholinesterase Paraoxon with Carboxylesterase Inhibitors. Biochemical. Pharmacology. 1998; 55(9): 1419–1426.Tuovinen K.,Kaliste-Korhonen E.,Hänninen O. Gender differences in activities of mouse esterase and sensitivities to DFP and sarin toxicity. Gen. Pharmacol. 1997; 29(3): 333-5.Tuovinen K.,Kaliste-Korhonen E.,Hänninen O. Gender differences in activities of mouse esterase and sensitivities to DFP and sarin toxicity. Gen. Pharmacol. 1997; 29(3): 333-5.Wehner J.M., Murphy-Erdosh C., Smolen A., Smolen T.N. Genetic variation in paraoxonase activity and sensitivity to diisopropylphosphofluoridate in inbred mice. Pharmacol. Biochem. Behav. 1987;28(2):317-20.Wehner J.M., Murphy-Erdosh C., Smolen A., Smolen T.N. Genetic variation in paraoxonase activity and sensitivity to diisopropylphosphofluoridate in inbred mice. Pharmacol. Biochem. Behav. 1987;28(2):317-20.Djeridane A., Yousfi M., Nadjemi B., Maamri S., Djireb F., Stocker P. Phenolic extracts from various Algerian plants as strong inhibitors of porcine liver carboxylesterase. J. Enzyme Inhib. Med. Chem. 2006; 21(6):719- 26.Djeridane A., Yousfi M., Nadjemi B., Maamri S., Djireb F., Stocker P. Phenolic extracts from various Algerian plants as strong inhibitors of porcine liver carboxylesterase. J. Enzyme Inhib. Med. Chem. 2006; 21(6):719- 26.Nomeir A.A., Abou-Sonia M.B. Studies on the metabolism of neurotoxic tri-o-cresyl phosphate. Toxicology. 1986; 38: 1-13.Nomeir A.A., Abou-Sonia M.B. Studies on the metabolism of neurotoxic tri-o-cresyl phosphate. Toxicology. 1986; 38: 1-13.

The authors declare that there are no conflicts of interest present.