toxreviewТоксикологический вестникToxicological Review0869-79223034-4611Federal Scientific Center of Hygiene named after F.F. Erisman10.36946/0869-7922-2020-6-3-13toxreview-346Research ArticleСтатьиПРОЯВЛЕНИЯ ПОДОСТРОЙ СИСТЕМНОЙ ТОКСИЧНОСТИ НАНОЧАСТИЦ ОКСИДА СВИНЦА ПРИ ИНГАЛЯЦИОННОЙ ЭКСПОЗИЦИИ КРЫСMANIFESTATIONS OF SUBACUTE SYSTEMIC TOXICITY OF LEAD OXIDE NANOPARTICLES IN RATS AFTER AN INHALATION EXPOSUREСутунковаМ. П.SutunkovaM. Р.

Сутункова Марина Петровна, доктор медицинских наук, директор 

620014, г. Екатеринбург

Sutunkova Marina Petrovna

620014, Yekaterinburg

marinasutunkova@yandex.ruСоловьёваС Н.SolovyevaS. N.

Соловьева Светлана Николаевна, научный сотрудник лаборатории токсикологии среды обитания 

620014, г. Екатеринбург

Solovyeva Svetlana Nikolaevna

620014, Yekaterinburg

solovyevasn@ymrc.ruЧернышовИ. Н.ChernyshovI. N.

Чернышов Иван Николаевич, младший научный сотрудник лаборатории токсикологии среды обитания

620014, г. Екатеринбург

Chernyshov Ivan Nikolaevich

620014, Yekaterinburg

chernishov@ymrc.ruКлиноваС. В.KlinovaS. V.

Клинова Светлана Владиславовна, научный сотрудник лаборатории промышленной токсикологии 

620014, г. Екатеринбург

Klinova Svetlana Vladislavovna

620014, Yekaterinburg

klinova.svetlana@gmail.comГурвичВ. Б.GurvichV. B.

Гурвич Владимир Борисович, доктор медицинских наук, научный руководитель центра 

620014, г. Екатеринбург

Gurvich Vladimir Borisovich

620014, Yekaterinburg

gurvich@ymrc.ruШурВ. Я.ShurV. Ya.

Шур Владимир Яковлевич, доктор физико-математических наук, профессор, директор, Уральский центр коллективного пользования «Современные нанотехнологии», Институт естественных наук и математики

620000, г. Екатеринбург

Shur Vladimir Yakovlevich

620000, Yekaterinburg

vladimir.shur@urfu.ruШишкинаЕ. В.ShishkinaE. V.

Шишкина Екатерина Владимировна, кандидат физико-математических наук, старший научный сотрудник Уральского центра коллективного пользования «Современные нанотехнологии», Институт естественных наук и математики

620000, Екатеринбург

Shishkina Ekaterina Vladimirovna

620000, Yekaterinburg

ekaterina.shishkina@labfer.usu.ruЗубаревИ. В.ZubarevI. V.

Зубарев Илья Владимирович, кандидат биологических наук, старший научный сотрудник института естественных наук и математики ременные нанотехнологии», Институт естественных наук и математики

620000, Екатеринбург

Zubarev Ilya Vladimirovich

620000, Yekaterinburg

ilyamitozubarev@gmail.comПриваловаЛ. И.PrivalovaL. I

Привалова Лариса Ивановна, доктор медицинских наук, профессор, зав. лабораторией научных основ биологической профилактики отдела токсикологии и биопрофилактики стественных наук и математики

620014, г. Екатеринбург

Privalova Larisa Ivanovna

620014, Yekaterinburg

Privalova@ymrc.ruКацнельсонБ. А.KatsnelsonB. A.

Кацнельсон Борис Александрович (Katsnelson Boris Alexandrovich), доктор медицинских наук, профессор, заслуженный деятель науки РФ, и.о. зав. отделом токсикологии и биопрофилактики 

620014, г. Екатеринбург

Katsnelson Boris Alexandrovich

620014, Yekaterinburg

bkaznelson@etel.ruФБУН «Екатеринбургский медицинский-научный центр профилактики и охраны здоровья рабочих промпредприятий» РоспотребнадзораРоссияYekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial WorkersRussian FederationИнститут естественных наук и математики, ФГАОУ ВО «Уральский Федеральный Университет имени первого Президента России Б.Н. Ельцина»РоссияInstitute of Natural Sciences, Ural Federal UniversityRussian Federation20200201202106313Copyright © Сутункова М.П., Соловьёва С.Н., Чернышов И.Н., Клинова С.В., Гурвич В.Б., Шур В.Я., Шишкина Е.В., Зубарев И.В., Привалова Л.И., Кацнельсон Б.А., 20212021Сутункова М.П., Соловьёва С.Н., Чернышов И.Н., Клинова С.В., Гурвич В.Б., Шур В.Я., Шишкина Е.В., Зубарев И.В., Привалова Л.И., Кацнельсон Б.А.Sutunkova M.Р., Solovyeva S.N., Chernyshov I.N., Klinova S.V., Gurvich V.B., Shur V.Y., Shishkina E.V., Zubarev I.V., Privalova L.I., Katsnelson B.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.toxreview.ru/jour/article/view/346

Белые аутбредные крысы-самки подвергались воздействию аэрозоля наночастиц оксида свинца в ингаляционной установке типа «только нос» при средней концентрации 1,30 ± 0,10 мг / м3 на протяжении 5 дней по 4 часа в день. Контрольную группу крыс параллельно подвергали псевдо-экспозиции в аналогичных условиях. Была обнаружена задержка наночастиц в легких и обонятельных луковицах головного мозга, продемонстрированная с помощью просвечивающей электронной микроскопии. Был выявлен ряд нарушений состояния организма в группе, подвергшейся воздействию, некоторые из которых можно считать показателями токсического действия, специфичными для свинца (увеличение доли ретикулоцитов, экскреции мочи с δ-аминолевулиновой кислотой), а другие – результатом проникновения наночастиц в мозг минуя гематоэнцефалический барьер (демиелинизация аксонов, вакуолизация цитоплазмы нейронов).

Outbred white female rats were exposed to an aerosol of lead oxide nanoparticles in a nose-only inhalation setup at an average concentration of 1.30 ± 0.10 mg / m3 during 5 days for 4 hours a day. In parallel, the control group of rats was subjected to sham exposure under similar conditions. The deposition of nanoparticles in the lungs and in the olfactory region of the brain was detected using transmission electron microscopy. Some disorders of the body state were detected in the exposed group, some of which can be considered as the result of a lead-specific toxic effect (an increase in the proportion of reticulocytes and in urinary excretion of δ-aminolevulinic acid), and others - the result of nanoparticles circumventing the blood-brain barrier (axon demyelination, vacuolization of the neurons’ cytoplasm).

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The authors declare that there are no conflicts of interest present.