toxreviewТоксикологический вестникToxicological Review0869-79223034-4611Federal Scientific Center of Hygiene named after F.F. Erisman10.36946/0869-7922-2021-29-3-50-55toxreview-429Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESМетодика электрохимического биотестирования в применении к сравнительной оценке антимикробных свойств эфирных маселElectrochemical biotesting technique as applied to comparative assessment of antimicrobial properties of essential oilshttps://orcid.org/0000-0003-0829-5213СибирцевВ. С.SibirtsevV. S.

Сибирцев Владимир Станиславович - кандидат химических наук, доцент ФГБОУ ВО СПбГХФУ МЗ РФ.

197376, Санкт-Петербург.

Vladimir S. Sibirtsev - PhD in Chemistry, Associate Professor, Saint Petersburg State Chemical Pharmaceutical University.

Saint Petersburg, 197376.

vs1969r@mail.ruhttps://orcid.org/0000-0002-4102-1129НечипоренкоУ. Ю.NechiporenkoU. Yu.

Нечипоренко Ульяна Юрьевна - младший научный сотрудник.

197376, Санкт-Петербург.

Ulyana Yu. Nechiporenko.

Saint Petersburg, 197376.

unechiporenko@yandex.ruhttps://orcid.org/0000-0001-9085-2984КабановВ. Л.KabanovV. L.

Кабанов Владимир Леонидович - младший научный сотрудник.

197376, Санкт-Петербург.

Vladimir L. Kabanov.

Saint Petersburg, 197376.

kabanof_v@yahoo.comhttps://orcid.org/0000-0003-1722-4644КукинМ. Ю.KukinM. Yu.

Кукин Михаил Юрьевич - кандидат технических наук, научный сотрудник.

196608, Санкт-Петербург.

Mikhail Yu. Kukin.

Saint Petersburg, 196608.

mk-1980_2@mail.ruСанкт-Петербургский государственный химико-фармацевтический университет Министерства здравоохранения Российской ФедерацииРоссияSaint Petersburg State Chemical Pharmaceutical UniversityRussian FederationСанкт-Петербургский филиал ФГАНУ «Научно-исследовательский институт хлебопекарной промышленности»РоссияScientific Research Institute of the Bakery IndustryRussian Federation202118072021035055Copyright © Сибирцев В.С., Нечипоренко У.Ю., Кабанов В.Л., Кукин М.Ю., 20212021Сибирцев В.С., Нечипоренко У.Ю., Кабанов В.Л., Кукин М.Ю.Sibirtsev V.S., Nechiporenko U.Y., Kabanov V.L., Kukin M.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.toxreview.ru/jour/article/view/429Введение

Введение. В последнее время всё более актуальной становится проблема разработки достаточно объективных, экспрессных и доступных для широкого применения способов оценки влияния различных химических соединений на динамику жизнедеятельности разных микроорганизмов; а из объектов тестирования всё больший интерес вызывают «эфирные масла» (ЭфМ), получаемые из различного растительного сырья.

Материал и методы

Материал и методы. В связи с вышесказанным, в этой работе описана методика биотестирования, предусматривающая периодическую (через каждые 2 ч) регистрацию изменений рН, редокс потенциала и электропроводности жидкой питательной среды, инкубируемой в присутствии и в отсутствие жизнеспособных тестовых микроорганизмов (ТМ) и тестируемых образцов (ТО). Результаты. С помощью представленной методики осуществлён сравнительный анализ антибиотической активности в отношении Staphylococcus aureus разных концентраций ЭфМ, полученных из 10 видов растительного сырья.

Заключение

Заключение. Как стало видно из полученных результатов, с помощью представленной методики можно существенно более экспрессно, объективно и информативно, чем при использовании стандартных визуальных методов микробиологического тестирования, оценивать влияние на динамику жизненной активности ТМ-образцов различной продукции. При этом биологическая активность ТО в отношении ТМ в большинстве случаев монотонно уменьшалась с увеличением времени их взаимодействия. А наиболее активные среди ТО пролонгированные антимикробные свойства в отношении ТМ проявили ЭфМ, полученные из листьев Thuja occidentalis, Eucalyptus globulus и Cupressus sempervirens.

A biotesting technique is described that provides for periodic (every 2 hours) recording of changes in pH, redox potential, and electrical conductivity of a liquid culture medium incubated in the presence and in the absence of viable test microorganisms (TM) and test samples (TS). The results of a comparative analysis using this technique of antibiotic activity against Staphylococcus aureus of different concentrations of «essential oils» obtained from 10 types of plant raw materials are presented. Based on this, we can conclude the following. Using the presented methodology, it is possible to assess the effect on the dynamics of the vital activity of TM of samples of various pharmaceutical, cosmetic, food, feed and other products, much more quickly, objectively and informatively than using standard visual methods of microbiological testing. The initial antibiotic activity of TS in most cases was greater than their prolonged antibiotic activity. At the same time, the mid-term (in terms of the time of interaction of TS with TM) antibiotic activity of TS was usually intermediate in value between their initial and prolonged biological activity.

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The authors declare that there are no conflicts of interest present.