toxreviewТоксикологический вестникToxicological Review0869-79223034-4611Federal Scientific Center of Hygiene named after F.F. Erisman10.36946/0869-7922-2016-6-18-24toxreview-50Research ArticleСтатьиНЕКОТОРЫЕ ЗАКОНОМЕРНОСТИ КОМБИНИРОВАННОЙ ТОКСИЧНОСТИ МЕТАЛЛООКСИДНЫХ НАНОЧАСТИЦSOME REGULATIES OF METAL OXIDE NPs COMBINED TOXICITYМинигалиеваИ. А.MinigalievaI. A.noemail@neicon.ruФБУН «Екатеринбургский медицинский-научный центр профилактики и охраны здоровья рабочих промпредприятий» РоспотребнадзораРоссияEkaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, RospotrebnadzorRussian Federation201628122016061824Copyright © Минигалиева И.А., 20162016Минигалиева И.А.Minigalieva I.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.toxreview.ru/jour/article/view/50

Т оксичность наночастиц (НЧ) оксидов нескольких металлов была изучена на аутбредных крысах-самках при изолированном и комбинированном (NiO+Mn3O4; CuO+PbO; CuO+ZnO; PbO+ZnO; PbO+CuO+ZnO) действии с использованием двух экспериментальных моделей: (а) однократная интратрахеальная инстилляция в малых дозах за 24 часа до проведения бронхоальвеолярного лаважа для цитологического и биохимического исследования получаемой жидкости; (б) повторные внутрибрюшинные инъекции на протяжении 6 недель в несмертельной дозировке, для оценки, вызванной этой субхронической интоксикацей по большому числу функциональных и морфометрических показателей и показателей био-распределения и элиминации соответствующих металлов. Комбинационные эффекты описывались различными математическими моделями, включая построение поверхности отклика. Было продемонстрировано многообразие типов комбинированной токсичности для одной и той же пары НЧ в зависимости от того, для какого конкретного эффекта она оценивается, а нередко также от дозо-зависимого уровня этого эффекта. Показано также, что риск-ориентированный подход к классификации трёхфакторной токсичности, ранее развитый для комбинированного действия металлов в ионно-молекулярной форме, адекватен и для изученных НЧ.

Toxicity of oxides nanoparticles (NPs) of some metals was studied in outbred female rats under isolated and combined action of (NiO + Mn3O4; CuO + PbO; CuO + ZnO; PbO + ZnO; PbO + CuO + ZnO) using two experimental models: ( a) a single intratracheal instillation in small doses 24 hours prior to the bronchoalveolar lavage procedure in cytology and biochemical investigations into the fluid produced; (b) repeated intraperitoneal injections at non-lethal dosage over 6 weeks to evaluate that sub-chronic intoxication produced using a great number of functional morphometric parameters and indicators of bio-distribution and elimination of the respective metals. Combination effects were described by various mathematical models including the construction of response surface. A variety of combined toxicity types was demonstrated for the same pair of NPs according to what concrete effect the toxicity was assessed and frequently also according to dose-dependent level of that effect. It was also shown that the risk-oriented approach to the classification of three-factor toxicity, previously developed for the combined action of metals in ion-molecular form, was adequate for NPs investigated,

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The authors declare that there are no conflicts of interest present.