toxreviewТоксикологический вестникToxicological Review0869-79223034-4611Federal Scientific Center of Hygiene named after F.F. Erismantoxreview-636Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИспользование сперматозоидов быка как экспресс-тест на митохондриальную токсичность Т-2 токсина и дельтаметринаUse of bovine spermatozoa as a rapid test for mitochondrial toxicity of T-2 toxin and deltamethrinhttps://orcid.org/0000-0001-6321-6130ТимербулатоваЛейсан МаратовнаTimerbulatovaLeisan Maratovna

младший научный сотрудник научно-исследовательского института физической культуры и спорта

Junior researcher of the research institute

L_Z_93@mail.ruПоволжский государственный университет физической культуры и спортаРоссияVolga Region State Academy of Physical Culture, Sport and TourismRussian Federation202321042023311636Copyright © Тимербулатова Л.М., 20232023Тимербулатова Л.М.Timerbulatova L.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.toxreview.ru/jour/article/view/636Введение

Введение. Современные требования по внедрению лекарственных препаратов в клиническую практику включают тест на митохондриальную токсичность. Митохондрии являются мишенями многих фармацевтических и терапевтических средств, которые могут их повредить и привести к изменениям в морфологии и функции. Сперматозоиды имеют одно из самых больших соотношений митохондрий к размеру тела, у них отсутствует цитоплазма между митохондриями и плазматической мембраной, поэтому они являются хорошей потенциальной моделью для экспресс-теста на митохондриальную токсичность.

Материал и методы

Материал и методы. Целью нашей работы стало исследование возможности использования   сперматозоидов  Bos taurus taurus для оценки митохондриальной токсичности. В качестве токсинов использовался Т-2 токсин и дельтаметрин. Основными изучаемыми параметрами были уровень митохондриального потенциала (с помощью красителя MitoTracker), подвижность сперматозоидов и их взаимосвязь.

Результаты

Результаты. Нами было обнаружено, что между подвижностью сперматозоидов быка и митохондриальным потенциалом их митохондрий существует сильная корреляция (R>0,87, p<0,05). Была подтверждена митохондриальная токсичность дельтаметрина, хотя и в гораздо меньшей степени, чем у Т-2 токсина. Кроме того, были обнаружены определенные закономерности в распределении активных зон митоходриального потенциала у сперматозоидов быка.

Заключение

Заключение. Таким образом, полученные нами результаты свидетельствуют о том, что митотоксичность может быть одним из основных факторов, опосредующих негативное влияние дельтаметрина на мужскую репродуктивную систему.

Introduction

Introduction. Modern requirements for the introduction of drugs into clinical practice include a test for mitochondrial toxicity. Mitochondria are targets of many pharmaceutical and therapeutic agents that can damage them and lead to changes in morphology and function. Spermatozoa have one of the largest ratios of mitochondria to body size, they lack the cytoplasm between the mitochondria and the plasma membrane, so they are a good potential model for a rapid test for mitochondrial toxicity.

Material and methods

Material and methods. The aim of our work was to study the possibility of using Bos taurus taurus spermatozoa to assess mitochondrial toxicity. T-2 toxin and deltamethrin were used as toxins. The main parameters studied were the level of mitochondrial potential (using the MitoTracker dye), sperm motility and their relationship.

Results

Results. We found that there is a strong correlation between the motility of bovine spermatozoa and the mitochondrial potential of their mitochondria (R>0.87, p<0.05). The mitochondrial toxicity of deltamethrin has been confirmed, although to a much lesser extent than that of the T-2 toxin. In addition, certain patterns were found in the distribution of active zones of the mitochondrial potential in bull spermatozoa.

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The authors declare that there are no conflicts of interest present.