toxreviewТоксикологический вестникToxicological Review0869-79223034-4611Federal Scientific Center of Hygiene named after F.F. Erisman10.47470/0869-7922-2023-31-6-363-375pdrhidtoxreview-783Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESСравнительный анализ цитологических показателей жидкости бронхоальвеолярного лаважа крыс после однократного воздействия металооксидных наночастицComparative analysis of cytology of rat bronchoalveolar lavage fluid after single exposure to metal oxide nanoparticleshttps://orcid.org/0000-0002-0927-4062КлиноваСветлана ВладиславовнаKlinovaSvetlana V.

Кандидат биологических наук, научный сотрудник ФБУН ЕМНЦ ПОЗРПП Роспотребнадзора, 620014, г. Екатеринбург, Российская Федерация

e-mail: klinova.svetlana@gmail.com

Researcher, Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers of Rospotrebnadzor, Yekaterinburg, Russian Federation

e-mail: klinova.svetlana@gmail.com

klinova.svetlana@gmail.comhttps://orcid.org/0000-0002-1743-7642СутунковаМарина ПетровнаSutunkovaMarina P.

Доктор медицинских наук, директор ФБУН «Екатеринбургский медицинский-научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, Екатеринбург, Россия

e-mail: sutunkova@ymrc.ru

MD, director of The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers of Rospotrebnadzor, 620014, Yekaterinburg, Russian Federation

e-mail: sutunkova@ymrc.ru

sutunkova@ymrc.ruhttps://orcid.org/0000-0002-0097-7845МинигалиеваИльзира АмировнаMinigalievaIlzira A.

LДоктор биологических наук, заведующая отделом токсикологии и биопрофилактики ФБУН «Екатеринбургский медицинский-научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, Екатеринбург, Россия

e-mail: ilzira-ilzira@ymrc.ru

Doctor of Biological Sciences, Head of the Department of Toxicology and Bioprevention of The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers of Rospotrebnadzor, 620014, Yekaterinburg, Russian Federation

e-mail: ilzira-ilzira@ymrc.ru  

ilzira-ilzira@ymrc.ruhttps://orcid.org/0000-0003-2677-0479РябоваЮлия ВладимировнаRyabovaYulia V.

Заведующая лабораторией научных основ биопрофилактики ФБУН «Екатеринбургский медицинский-научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, Екатеринбург, Россия

e-mail: ryabova@ymrc.ru  

Head of the Laboratory of Scientific Fundamentals of Bioprevention of The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers of Rospotrebnadzor, 620014, Yekaterinburg, Russian Federation

e-mail: ryabova@ymrc.ru

ryabova@ymrc.ruhttps://orcid.org/0000-0001-9384-8550ТажигуловаАнастасия ВалерьевнаTazhigulovaAnastasiya V.

Младший научный сотрудник лаборатории научных основ биопрофилактики ФБУН «Екатеринбургский медицинский-научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, Екатеринбург, Россия

e-mail: tazhigulovaav@ymrc.ru

Junior Researcher, Laboratory of Scientific Fundamentals of Bioprevention of The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers of Rospotrebnadzor, 620014, Yekaterinburg, Russian Federation

e-mail: tazhigulovaav@ymrc.ru  

tazhigulovaav@ymrc.ruhttps://orcid.org/0000-0002-1442-6737ПриваловаЛариса ИвановнаPrivalovaLarisa I.

Доктор медицинских наук, профессор, главный научный сотрудник отдела токсикологии и биопрофилактики ФБУН «Екатеринбургский медицинский-научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, Екатеринбург, Россия

e-mail: privalovali@yahoo.com

MD, professor, Chief Researcher of Department of Toxicology and Bioprevention of The Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial Workers of Rospotrebnadzor, 620014, Yekaterinburg, Russian Federation

e-mail: privalovali@yahoo.com 

privalovali@yahoo.comФБУН «Екатеринбургский медицинский-научный центр профилактики и охраны здоровья рабочих промпредприятий» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человекаРоссияThe Yekaterinburg Medical Research Center for Prophylaxis and Health Protection of Industrial WorkersRussian Federation202315012024316363375Copyright © Клинова С.В., Сутункова М.П., Минигалиева И.А., Рябова Ю.В., Тажигулова А.В., Привалова Л.И., 20242024Клинова С.В., Сутункова М.П., Минигалиева И.А., Рябова Ю.В., Тажигулова А.В., Привалова Л.И.Klinova S.V., Sutunkova M.P., Minigalieva I.A., Ryabova Y.V., Tazhigulova A.V., Privalova L.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.toxreview.ru/jour/article/view/783Введение

Введение. Производственная деятельность человека (металлургическая и горнодобывающая промышленность, производство и переработка электроники, аккумуляторов) связана с загрязнением воздуха рабочей зоны и среды обитания аэрозолями сложного состава. Наиболее опасными среди компонентов таких аэрозолей считаются ультрамалые частицы нанометрового диапазона, в том числе нанооксиды металлов. В связи с их распространенностью исследования по оценке цитотоксичности металлооксидных наночастиц являются актуальными.

Материал и методы

Материал и методы. Суспензии наночастиц (НЧ) CuO, PbO, CdO, Fe2O3, NiO были получены методом лазерной абляции. Исследования проводились на белых аутбредных крысах-самках. Проведена однократная интратрахеальная инстилляция НЧ различной химической природы в дозе 0,5 мг/животное; контрольные животные получали аналогичный объем деионизированной воды. Через 1 сут после введения НЧ у животных проводился бронхоальвеолярный лаваж (БАЛ) с последующей оценкой его цитологических показателей.

Результаты

Результаты. Цитотоксическое действие исследованных НЧ, исходя из цитологических параметров жидкости БАЛ, изменяется следующим образом (от большего к меньшему): НЧ CuO > НЧ CdO > НЧ PbO > НЧ NiO > НЧ Fe2O3.

Ограничения исследования

Ограничения исследования. Такие физические характеристики наночастиц, как растворимость в воде и биологических жидкостях, заряд, адсорбционная способность, устойчивость к агрегации, гидрофобность, адгезия к поверхности, способность генерировать свободные радикалы, не были изучены. Экстраполяцию данных от грызунов на человека следует проводить с осторожностью, поскольку цитотоксичность охарактеризована только на основе цитологических показателей.

Заключение

Заключение. Таким образом, сравнительный анализ изменений цитологических показателей бронхоальвеолярного лаважа может быть использован как эффективный скрининговый метод цитотоксического действия НЧ.

Соблюдение этических стандартов. Работа одобрена Локальным независимым этическим комитетом ФБУН ЕМНЦ ПОЗРПП Роспотребнадзора (протокол № 2 от 20.04.2020).

Участие авторов

Участие авторов:Сутункова М.П., Минигалиева И.А., Привалова Л.И. — концепция и дизайн исследования, редактирование;Клинова С.В., Рябова Ю.В. — проведение эксперимента, сбор и обработка материала, написание текста, редактирование;Тажигулова А.В. — проведение эксперимента, сбор и обработка материала, редактирование.Все соавторы — утверждение окончательного варианта статьи, ответственность за целостность всех частей статьи.

Благодарность

Благодарность. Авторы выражают благодарность коллективу Уральского центра коллективного пользования «Современные нанотехнологии» Уральского федерального университета имени первого Президента России Б.Н. Ельцина и лично директору УЦКП СН, профессору, д.ф-м.н. Шуру Владимиру Яковлевичу за синтез суспензий, исследованных наночастиц заданной характеристики на базе УЦКП СН УрФУ.

Конфликт интересов

Конфликт интересов. Авторы заявляют, что у них нет конфликта интересов.

Финансирование

Финансирование. Исследование не имело спонсорской поддержки.

Дата поступления

Дата поступления: 18 мая 2023 / Дата принятия в печать: 03 декабря 2023 / Дата публикации: 29 декабря 2023

 

 

Introduction

Introduction. Human production activities (metallurgical, mining, electronics production and processing, batteries) are related to air pollution of the working area and the environments of complex composition aerosols. Among the aerosol components, ultrathin particles of the nanometer range (including metal nano-oxides) are considered to be the most dangerous. Due to their prevalence, study to assess the cytotoxicity of metal oxide nanoparticles are relevant.

Material and methods

Material and methods. CuO, PbO, CdO, Fe2O3, NiO nanoparticle (NP) suspension were obtained by laser ablation. The study was done on white outbred female rats. A single intratracheal instillation of different chemical NPs was performed (in dose 0.5 mg/animal); control animals received a similar amount of deionized water. A day after the NP instillation bronchoalveolar lavage (BAL) was carried out with the subsequent assessment of its cytological indices.

Results

Results. The cytotoxic action of the studied NPs, based on the cytological indices of the BAL fluid, is changed as follows (from greater to lesser): CuO NP > CdO NP > PbO NP > NiO NP > Fe2O3 NP.

Limitations

Limitations. Such physical characteristics of nanoparticles as solubility in water and biological fluids, charge, adsorption capacity, resistance to aggregation, hydrophobicity, adhesion to surfaces, and the ability to generate free radicals have not been studied. Extrapolation of data from rodents to humans shall be done with caution, since cytotoxicity has been characterized only based on the main cellular parameters.

Conclusion

Conclusion. Bronchoalveolar lavage cytology can be used as an effective screening method for the cytotoxic effect of NPs.

Compliance with ethical standards. The animal study protocols were approved by the Institutional Ethics Committee of the Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers (Protocol No. 2 of April 20, 2020).

Contribution of the authors:Sutunkova M.P., Minigalieva I.A., Privalova L.I. — the concept and design of the study, editing;Klinova S.V., Ryabova Yu.V. — conducting a study, the collection and processing of the material, writing text, editing;Tazhigulova A.V. — conducting a study, the collection and processing of the material, editing.All co-authors — approval of the final version of the article, responsibility for the integrity of all parts of the article.

Acknowledgment

Acknowledgment. Authors express their gratitude to the team of the Ural Center for Collective Use “Modern Nanotechnologies” of the Ural Federal University named after the first President of Russia B.N. Yeltsin and personally to the director of the Ural Center for Collective Use “Modern Nanotechnologies”, professor, doctor of physical and mathematical sciences Vladimir Yakovlevich Shur, for the synthesis of suspensions, studied nanoparticles of a given characteristic on the basis of the “Modern Nanotechnologies” center.

Conflict of interests

Conflict of interests. The authors declare no conflict of interests.

Funding

Funding. The study had no sponsorship.

Date of receipt

Date of receipt: May 18, 2023 / Date of acceptance for printing: December 3, 2023 / Date of publication: December 29, 2023

 

 

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The authors declare that there are no conflicts of interest present.