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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">toxreview</journal-id><journal-title-group><journal-title xml:lang="ru">Токсикологический вестник</journal-title><trans-title-group xml:lang="en"><trans-title>Toxicological Review</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0869-7922</issn><issn pub-type="epub">3034-4611</issn><publisher><publisher-name>Federal Scientific Center of Hygiene named after F.F. Erisman</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.36946/0869-7922-2017-4-50-54</article-id><article-id custom-type="elpub" pub-id-type="custom">toxreview-87</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>ОЦЕНКА ТОКСИЧНОСТИ ТЕХНОГЕННЫХ НАНОЧАСТИЦ C ИСПОЛЬЗОВАНИЕМ ВОДОРОСЛИ CHLORELLA VULGARIS</article-title><trans-title-group xml:lang="en"><trans-title>TOXICITY ASSESSMENT OF ENGINEERED NANOPARTICLES USING ALGAE CHLORELLA VULGARIS</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Асанова</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Asanova</surname><given-names>A. A.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Полонский</surname><given-names>В. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Polonskiy</surname><given-names>V. I.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Григорьев</surname><given-names>Ю. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Grigoriev</surname><given-names>Y. S.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБОУ ВО Красноярский государственный аграрный университет, Институт агроэкологических технологий</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Krasnoyarsk State Agricultural University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФГАОУ ВО Сибирский федеральный университет, Институт экологии и географии</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Siberian Federal University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2017</year></pub-date><pub-date pub-type="epub"><day>28</day><month>08</month><year>2017</year></pub-date><volume>0</volume><issue>4</issue><fpage>50</fpage><lpage>54</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Асанова А.А., Полонский В.И., Григорьев Ю.С., 2017</copyright-statement><copyright-year>2017</copyright-year><copyright-holder xml:lang="ru">Асанова А.А., Полонский В.И., Григорьев Ю.С.</copyright-holder><copyright-holder xml:lang="en">Asanova A.A., Polonskiy V.I., Grigoriev Y.S.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.toxreview.ru/jour/article/view/87">https://www.toxreview.ru/jour/article/view/87</self-uri><abstract><p>Применение наночастиц с каждым годом охватывает новые сферы производства. Их проникновение в окружающую среду возможно на любом этапе существования: от процесса изготовления до утилизации в составе конечного продукта, поэтому вероятные риски для различных объектов окружающей среды должны быть оценены. Целью настоящей работы является оценка влияния техногенных наночастиц на рост и фотосинтетическую активность Chlorella vulgaris. Исследованы токсические свойства трех видов наночастиц: TiO2 размером 100-190 нм, SiO2 размерами 10-15 нм и 100-120 нм. В качестве тест-организма использовали водоросль Chlorella vulgaris Beijer. Оценку токсичности наночастиц проводили по изменению относительного показателя замедленной флуоресценции (ОПЗФ), характеризующего фотосинтетическую активность микроводоросли. Воздействие на рост тест-культуры определяли по величине оптической плотности (ОП) водорослевой суспензии. Было установлено, что наночастицы TiO2 незначительно снижали рост и активность фотосинтетического аппарата клеток. Крупные частицы SiO2 оказались более токсичными, чем частицы меньшего размера. Для наночастиц 100- 120 нм величина ЕС50 по показателю ОП составила 8 мг/дм3, тогда как частицы размером 10-15 нм не проявляли заметного влияния на рост культуры. По параметру ОПЗФ крупные частицы SiO2 также оказались более токсичными, чем мелкие.</p></abstract><trans-abstract xml:lang="en"><p>The use of nanoparticles covers new spheres of production every year. Their release into the environment is possible at any stage of their lifespan: from the manufacturing process to the utilization in the final product; therefore potential risks they pose for various environmental objects should be evaluated. The purpose of this work was to assess the effect of technogenic nanoparticles on Chlorella vulgaris growth and photosynthetic activity. Toxic properties of three types of nanoparticles TiO2 with a size of 100 to 190 nm, SiO2 with dimensions of 10-15 nm and 100-120 nm have been studied. Chlorella vulgaris Beijer alga was used as test organism. The evaluation of nanoparticles toxicity was based on changes in the relative delayed fluorescence index (RDFI), which characterizes the photosynthetic activity of microalgae. The effect on the growth of the test culture was determined from the optical density (OD) of the algal suspension. It was found that the TiO2 nanoparticles slightly reduced the growth and activity of the cell photosynthetic apparatus. Large particles of SiO2 proved to be more toxic than smaller ones. For nanoparticles of 100-120 nm size , the EC50 value according to the OD index was 8 mg/dm3, while the particles measuring 10-15 nm did not show a significant effect on the growth of the culture. Large SiO2 particles also appeared more toxic than small particles, judging by RDFI parameter.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>наночастицы</kwd><kwd>нанотоксичность</kwd><kwd>Chlorella vulgaris</kwd><kwd>биотестирование</kwd><kwd>замедленная флуоресценция хлорофилла</kwd><kwd>оптическая плотность</kwd></kwd-group><kwd-group xml:lang="en"><kwd>nanoparticles</kwd><kwd>nanotoxicity</kwd><kwd>Chlorella vulgaris</kwd><kwd>biotesting</kwd><kwd>delayed fluorescence of chlorophyll</kwd><kwd>optical density</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Kessler R. Engineered nanoparticles in consumer products: understanding a new ingredient. Environ Health Perspect. 2011; 119 (3): A120-A1</mixed-citation><mixed-citation xml:lang="en">Kessler R. Engineered nanoparticles in consumer products: understanding a new ingredient. 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