toxreviewТоксикологический вестникToxicological Review0869-79223034-4611Federal Scientific Center of Hygiene named after F.F. Erisman10.36946/0869-7922-2017-5-35-43toxreview-93Research ArticleСтатьиНекоторые аспекты оценки токсичности металЛо- оксидных наночастиц на клеточных культурах (на примере NiO и Mn3O4)Some aspects of metal oxide nanoparticles toxicity assessment on cell cultures as exemplified by NiO and Mn3O4МинигалиеваИ. А.MinigalievaI. А.noemail@neicon.ruБушуеваТ. В.BushuevaT. V.noemail@neicon.ruПановВ. Г.PanovV. G.noemail@neicon.ruВараксинА. Н.VaraksinA. N.noemail@neicon.ruШурВ. Я.ShurV. Ya.noemail@neicon.ruШишкинаЕ. В.ShishkinaE. V.noemail@neicon.ruГурвичВ. Б.GurviсhV. B.noemail@neicon.ruКацнельсонБ. А.KatsnelsonB. A.noemail@neicon.ruФБУН «Екатеринбургский медицинский научный центр профилактики и охраны здоровья рабочих промпредприятий» РоспотребнадзораРоссияMedical Research Center for Prophylaxis and Health Protection in Industrial WorkersRussian FederationИнститут промышленной экологии УрО РАНРоссияInstitute of Industrial Ecology, the Urals Branch of the Russian Academy of SciencesRussian FederationИнститут естественных наук и математики ФГАОУ ВО «УрФУ имени первого Президента России Б.Н. Ельцина»РоссияSchool of Natural Sciences and Mathematics, the Ural Federal UniversityRussian Federation201728102017053543Copyright © Минигалиева И.А., Бушуева Т.В., Панов В.Г., Вараксин А.Н., Шур В.Я., Шишкина Е.В., Гурвич В.Б., Кацнельсон Б.А., 20172017Минигалиева И.А., Бушуева Т.В., Панов В.Г., Вараксин А.Н., Шур В.Я., Шишкина Е.В., Гурвич В.Б., Кацнельсон Б.А.Minigalieva I.А., Bushueva T.V., Panov V.G., Varaksin A.N., Shur V.Y., Shishkina E.V., Gurviсh V.B., Katsnelson B.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.toxreview.ru/jour/article/view/93

Сравнительная и комбинированная оценка повреждающего действия наночастиц NiO и Mn3O4 получена на культурах различных человеческих стабильных клеточных линий. Найдено, что добавление эмбриональной бычьей сыворотки крови (FBS) ко всем использованным культуральным средам делает эти металлооксидные наночастицы (MeO-НЧ), в особенности, Mn3O4-НЧ экспоненциально растворимыми, в то время как без FBS их растворимость крайне низка. Вместе с тем, присутствие FBS существенно замедляет седиментацию, связанную с агрегацией этих MeO-НЧ. Показана зависимость повреждения клеток от концентрации МеО-НЧ при более высокой цитотоксичности Mn3O4-НЧ по сравнению с NiO-НЧ. Таким образом, сравнительная оценка неспецифической токсичности, полученная ранее в экспериментах «ин виво», воспроизведена «in vitro». Однако по отношению к ранее обнаруженному марганец-специфичному повреждению головного мозга при субхронической интоксикации теми же наночастицами, нынешний эксперимент на нейронах «in vitro» показал лишь некоторое усиливающее влияние Mn3O4-НЧ в комбинации с NiO-НЧ, роль которых преобладала.

Comparative and combined damaging actions of NiO and Mn3O4 anoparticles were estimated on cultures of different established human cell lines. It was found out that the addition of the fetal bovine serum (FBS) to the culture media ,used in the investigation, renders NiO-NPs and, to even a greater extent, Mn3O4-NPs exponentially soluble while without FBS their dissolution was extremely low. Along with it, sedimentation of those MeO-NPs caused by their aggregation noticeably slowed down in the presence of the same FBS. The dependence of cell damage on the MeO-NPs concentration was found out, at a higher cytotoxicity of Mn3O4-NP as compared to NiO-NP. Thus, comparative assessment of NPs non-specific toxicity previously obtained in animal experiments was reproduced in the «in vitro» tests. However, with respect to manganese-specific brain damage «in vivo» discovered previously in sub-chronic intoxication with the same MeO-NPs, the present «in vitro» experiment on neurons only showed a certain enhancing effect of Mn3O4-NP on the action of NiO-NP, but the role of NiO-NP in the combination prevailed.

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The authors declare that there are no conflicts of interest present.