ВЛИЯНИЕ СУБХРОНИЧЕСКОЙ СВИНЦОВОЙ ИНТОКСИКАЦИИ НА СОКРАТИМОСТЬ МИОКАРДА КРЫСЫ

Известно, что хроническая свинцовая интоксикация вызывает артериальную гипертензию как у людей, так и у крыс и, таким образом, является причиной некоторых вторичных на-рушений функции сердца. Однако нет никаких неопровержимых доказательств того, что сама интоксикация непосредственно влияет на сократимость миокарда. В наших экспериментах аутбредным крысам-самцам многократно внутрибрюшинно вводили сублетальные дозы ацетата свинца 3 раза в неделю в течение 5 недель. Мы наблюдали явную, хотя и умеренную субхроническую свинцовую интоксикацию. На следующий день после последней инъекции сердце каждого животного было извлечено; трабекулы и папиллярные мышцы из правого желудочка были выделены для моделирования цикла сокращения-расслабления in vitro в изометрических режимах; оставшаяся ткань сердца гомогенизировалась для определения изоформ миозина. Было обнаружено, что свинцовая интоксикация приводила к снижению (по сравнению с контрольными крысами) времени и скорости изометрического сжатия препаратов миокарда in vitro, в то время как сохранялась его амплитуда и уменьшалось пассивное напряжение трабекул. Показано, что отношение миозина сдвигалось в сторону медленной изоформы. Обсуждаются механические и токсикологические выводы из полученных результатов.

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