STUDY OF THE EFFECTIVENESS OF RECOMBINANT PLASMIDS IN A NEUROPATHY MODEL AT POISONING WITH MALATHION
Abstract
The effectiveness of gene therapy agents application, i.e.: recombinant plasmid DNA (RPD) coding body growth factor production (nerve growth factor (NGF), vascular endothelial growth factor (VEGF) and somatoliberin-1) was shown using a model of peripheral neuropathy caused by a thrice-repeated 0.5 LD50 malathion injection to rats. RPD NGF and RPD VEGF were found to have the strongest nerve tissue protective effect. According to electrophysiological studies findings, the preparations based on these plasmid constructions exerted a positive effect as early as a week after the damage and completely removed neuropathy manifestations by the 21 day.
About the Authors
Viktor Vasil’evich Shilov
Northwest Scientific Center of Hygiene and Public Health
Russian Federation
Russian Federation
Mikhail Anatol’evich Judin
Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy
Russian Federation
Russian Federation
Taras Vladimirovich Shherbakov
Northwest Scientific Center of Hygiene and Public Health
Russian Federation
Russian Federation
Olga Alexandrovna Belichenko
Northwest Scientific Center of Hygiene and Public Health
Russian Federation
Russian Federation
References
1. Kucenko S.A. Osnovy toksikologii. 2002; 327-328 (in Russian).
2. Kucenko S.A. Voennaja toksikologija, radiobiologija i medicinskaja zashhita. 2004 (in Russian).
3. Rinsho S. Polyneuropathy: diagnosis and treatment. 2007; Vol.47, №11, 769-773.
4. Grogan P.M., Katz J.S. Toxic neuropathies /NeurolClin. 2005; Vol. 23, № 2, P. 377-396.
5. Mzhavanadze N.D., Bozo I.Ja., Kalinin R.E., Deev R.V. Realii i perspektivy primenenja gennoj terapii v serdechno-sosudistoj hirurgii. Kletochnaja transplantologja i kletochnaja inzhenerja. 2013; Tom VII, №2 (in Russian).
6. Nikolaev S.G. Praktikum po klinicheskoj jelektromiografii. 2003 (in Russian).
7. Prozorovskij V. B., Skopichev V. G., Ardab’eva T.V. i dr. Morfologicheskie izmenenja jeritrocitov kak vozmozhnaja prichina nizkoj jeffektivnosti specificheskoj terapii pri otravlenii karbofosom.Morfologja, 1997.-T. 112.-№6.-S-60-64 (in Russian).
8. Mironov A.N., Bunjatjan N.D., Uteshev D.B., Radaev S.M. i dr. Sostojanie i perspektivy razvitja genoterapii v Rossii. Vestnik Roszdravnadzora. 2011, №4 (in Russian).
9. Deev R.V., Kalinin R.E., Chervjakov Ju.V., Grjaznov S.V., Mzhavanadze N.D. i dr. Rezul’taty primenenija genterapevticheskogo preparata «Neovaskulgen» u pacientov s hronicheskoj ishemiej nizhnih konechnostej: 1 god nabljudenij. Vestnik Nacional’nogo mediko-hirurgicheskogo Centra im.N.I.Pirogova. 2011, T.6, №4 (in Russian).
10. Sondell M., Lundborg G., Kanje M. Vascular endothelial growth factor stimulates Schwann cell invasion and neovascularization of acellular nerve grafts // Brain Res. - 1999 Nov 6. - Vol. 846, № 2. - Р. 219-28.
11. Masgutov R.F., Rizvanov A.A., Bogov A.A. (ml.), Galljamov A.R. i dr. Sovremennye tendencii lechenja perifericheskih nervov. Nevrologja, prakticheskaja medicina 1-1 (13). Aktual’nye problemy mediciny. 2013. T.2 (in Russian).
Review
For citations:
Shilov V.V., Judin M.A., Shherbakov T.V., Belichenko O.A. STUDY OF THE EFFECTIVENESS OF RECOMBINANT PLASMIDS IN A NEUROPATHY MODEL AT POISONING WITH MALATHION. Toxicological Review. 2015;(4):26-30. (In Russ.)
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