Некоторые аспекты оценки токсичности металЛо- оксидных наночастиц на клеточных культурах (на примере NiO и Mn3O4)

Сравнительная и комбинированная оценка повреждающего действия наночастиц NiO и Mn3O4 получена на культурах различных человеческих стабильных клеточных линий. Найдено, что добавление эмбриональной бычьей сыворотки крови (FBS) ко всем использованным культуральным средам делает эти металлооксидные наночастицы (MeO-НЧ), в особенности, Mn3O4-НЧ экспоненциально растворимыми, в то время как без FBS их растворимость крайне низка. Вместе с тем, присутствие FBS существенно замедляет седиментацию, связанную с агрегацией этих MeO-НЧ. Показана зависимость повреждения клеток от концентрации МеО-НЧ при более высокой цитотоксичности Mn3O4-НЧ по сравнению с NiO-НЧ. Таким образом, сравнительная оценка неспецифической токсичности, полученная ранее в экспериментах «ин виво», воспроизведена «in vitro». Однако по отношению к ранее обнаруженному марганец-специфичному повреждению головного мозга при субхронической интоксикации теми же наночастицами, нынешний эксперимент на нейронах «in vitro» показал лишь некоторое усиливающее влияние Mn3O4-НЧ в комбинации с NiO-НЧ, роль которых преобладала.

оксид марганца, оксид никеля, наночастицы, токсичность «in vitro»

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