Some aspects of metal oxide nanoparticles toxicity assessment on cell cultures as exemplified by NiO and Mn3O4
https://doi.org/10.36946/0869-7922-2017-5-35-43
Abstract
Comparative and combined damaging actions of NiO and Mn3O4 anoparticles were estimated on cultures of different established human cell lines. It was found out that the addition of the fetal bovine serum (FBS) to the culture media ,used in the investigation, renders NiO-NPs and, to even a greater extent, Mn3O4-NPs exponentially soluble while without FBS their dissolution was extremely low. Along with it, sedimentation of those MeO-NPs caused by their aggregation noticeably slowed down in the presence of the same FBS. The dependence of cell damage on the MeO-NPs concentration was found out, at a higher cytotoxicity of Mn3O4-NP as compared to NiO-NP. Thus, comparative assessment of NPs non-specific toxicity previously obtained in animal experiments was reproduced in the «in vitro» tests. However, with respect to manganese-specific brain damage «in vivo» discovered previously in sub-chronic intoxication with the same MeO-NPs, the present «in vitro» experiment on neurons only showed a certain enhancing effect of Mn3O4-NP on the action of NiO-NP, but the role of NiO-NP in the combination prevailed.
About the Authors
I. А. Minigalieva
Medical Research Center for Prophylaxis and Health Protection in Industrial Workers
Russian Federation
Russian Federation
T. V. Bushueva
Medical Research Center for Prophylaxis and Health Protection in Industrial Workers
Russian Federation
Russian Federation
V. G. Panov
Institute of Industrial Ecology, the Urals Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
A. N. Varaksin
Institute of Industrial Ecology, the Urals Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
V. Ya. Shur
School of Natural Sciences and Mathematics, the Ural Federal University
Russian Federation
Russian Federation
E. V. Shishkina
School of Natural Sciences and Mathematics, the Ural Federal University
Russian Federation
Russian Federation
V. B. Gurviсh
Medical Research Center for Prophylaxis and Health Protection in Industrial Workers
Russian Federation
Russian Federation
B. A. Katsnelson
Medical Research Center for Prophylaxis and Health Protection in Industrial Workers
Russian Federation
Russian Federation
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For citations:
Minigalieva I.А., Bushueva T.V., Panov V.G., Varaksin A.N., Shur V.Ya., Shishkina E.V., Gurviсh V.B., Katsnelson B.A. Some aspects of metal oxide nanoparticles toxicity assessment on cell cultures as exemplified by NiO and Mn3O4. Toxicological Review. 2017;(5):35-43. (In Russ.) https://doi.org/10.36946/0869-7922-2017-5-35-43
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