ACTIVITY OF APOPTOSIS IN THE NERVE TISSUE OF WHITE RATS EXPOSED TO NANOSILVER ARABINOGALACTAN
Abstract
The article presents results of apoptosis evaluation in albino rat neurons using comet assay for DNA and immune histochemical analysis to determine the activity of pro-and anti-apoptotic proteins caspase 3 and bcl-2 at exposure to nano biocomposites representing nano silver embedded in a matrix of a natural arabinogalactan. In preparations of nervous tissue of rats exposed to nano silver arabinogalactan polymer, a statistically significant increase of DNA content in comet tails was found out. DNA comets with DNA tail content of more than 70%, which could be regarded as apoptotic have not been observed in investigated formulations. The results of the immune histochemical determination of expression of anti-and pro- apoptotic proteins bcl-2 and caspase-3 in neurons show on the contrary the development of apoptosis in nerve cells. Comparing results of the studies performed, a conclusion can be drawn that to detect the apoptosis process, immune histochemical methods for determination of anti-and pro-apoptotic proteins are more efficient than DNA comet assay.
Keywords
About the Authors
Larisa Mikhailovna Sosedova
East-Siberian Institute of Medico-Ecological Researches
Russian Federation
Russian Federation
Ekaterina Aleksandrovna Kapustina
East-Siberian Institute of Medico-Ecological Researches
Russian Federation
Russian Federation
Mikhail Aleksandrovich Novikov
East-Siberian Institute of Medico-Ecological Researches
Russian Federation
Russian Federation
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Review
For citations:
Sosedova L.M., Kapustina E.A., Novikov M.A. ACTIVITY OF APOPTOSIS IN THE NERVE TISSUE OF WHITE RATS EXPOSED TO NANOSILVER ARABINOGALACTAN. Toxicological Review. 2015;(6):27-31. (In Russ.)
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