DETERMINATION OF the TOXICITY CLASS OF A GRATIOLA OFFICINALIS L. PLANT EXTRACT, USING LARVAE CHIRONOMUS RIPARIUS AS A NEW TEST OBJECT
Abstract
The herb golden pert (Gratiola Officinalis ) was used as starting material in the study. Magnitudes of the median lethal concentration (LC50) and accumulation character determined in larvae Chironomus riparius were taken as toxicity indicators on the organismal level. Results of the extract toxicity obtained with larvae were compared with those obtained with laboratory mice in terms of median lethal dose (LD50). A possibility of using Chironomid larvae Ch.riparius as a new test object in pre-clinical safety assessment of pharmacological substances was proved. A semi-lethal concentration (LC50) of the bio flavonoid extract in which double-wing insects larvae Chironomid Ch.riparius were put was 2280 mg/l. A semi-lethal dose (LD50) determined in laboratory mice at intra-abdominal administration was equal to 2375 mg/l. According to the toxicity classification of substances, the extract refers to the class of practically non- toxic substances. The herb golden pert extract has a weak ability of accumulation (TL50<12).
About the Authors
Maria Nikolaevna Kurchatova
State Budgetary Educational Institution V.I. Razumovsky «Saratov State Medical University». RF Ministry of Health of Russia
Russian Federation
Russian Federation
Natalia Vladimirovna Polukonova
State Budgetary Educational Institution V.I. Razumovsky «Saratov State Medical University». RF Ministry of Health of Russia
Russian Federation
Russian Federation
Natalia Anatolievna Durnova
State Budgetary Educational Institution V.I. Razumovsky «Saratov State Medical University». RF Ministry of Health of Russia
Russian Federation
Russian Federation
References
1. Navolokin N.A., Polukonova N.V., Maslyakova G.N., Bucharskaya A.B., Durnova N.A. Effect of extracts of Gratiola officinalis and Zea mays on the tumor and the morphology of the internal organs of rats with transplanted liver cancer // LLC Science and Innovations, Saratov, Russia. 2012. P. 1-4.
2. Korosov A.V., Kalinkina N.M. Quantitative methods in environmental toxicology: textbook // PetrGU, KNC. Petrozavodsk; 2003. 56 p. (in Russian)
3. Kurkin V.A. Pharmacognosy: a textbook for pharmaceutical universities (faculties) // V.A. Kurkin. 2-e izd., pererab. i dop. Samara; 2007. 1239 p. (in Russian)
4. Methods for assessing the ecological risks of pesticides in registration (Manual of classifications of ecological risks of pesticides) Rossijskaja Akademija Sel’skohozjajstvennyh Nauk Gosudarstvennoe nauchnoe uchrezhdenie Vserossjskj nauchno-issledovatel’skj institut fitopatologii (VNIIF) Bol’shie Vjazemy. 2010. (in Russian)
5. MU 2.1.5.720—98 Substantiation of hygienic standards for chemical substances in water of water bodies of drinking and household water use (NII jekologii cheloveka i gigieny okruzhajushhej sredy im. A.N. Sysina RAMN). (in Russian)
6. «Evaluation of the toxicity and dangers of disinfectants» Methodical instructions MU 1.2.1105-02 (utv. Glavnym gosudarstvennym sanitarnym vrachom RF 10 fevralja 2002.) (in Russian)
7. Patent № 2482863, A method of obtaining a dry extract from vegetable raw materials with biological activity / Polukonova N.V., Navolokin N.A., Durnova N.A., Masljakova G.N., Bucharskaja A.B.// Zajavka na patent № 2012105384, prioritet ot 15.02.2012. Polozhitel’noe reshenie ot 7.02.2013 (in Russian).
8. RD 52.24.635-2002. Methodical instructions. Check observations to assess the level of toxic pollution of bottom sediments on the basis of biotesting. Methods for Toxicological assessment of pollution of freshwater ecosystems. Moscow: Rosgidromet. 15 p. (in Russian)
9. The guidelines for conducting pre-clinical studies of drugs. ch. 1 // Pod red. A.N. Mironova. Moscow: Grif i K; 2012. 944 p. (in Russian)
10. Fedorova I.A., Polukonova N.V., Dvoreckij K.N., Bogoslovskaja S.I. The functional activity of polytene chromosomes of Chironomus (Diptera) under the influence of chalinochromis drugs atropine and pilocarpine // Jekologicheskaja genetika. 2009. T. 7 (№3). P. 44-52 (in Russian).
11. Fedorova I.A., Polukonova N.V., Petrov N.V. Cytogenetic effects of chalinochromis drugs with combined effect on larvae of Chironomus plumosus (DIPTERA) in vivo // CitologUa, 2009, Tom 51 (№10). P. 849855 (in Russian).
Review
For citations:
Kurchatova M.N., Polukonova N.V., Durnova N.A. DETERMINATION OF the TOXICITY CLASS OF A GRATIOLA OFFICINALIS L. PLANT EXTRACT, USING LARVAE CHIRONOMUS RIPARIUS AS A NEW TEST OBJECT. Toxicological Review. 2014;(6):40-43. (In Russ.)
Views:
263
Email this article 