EVALUATION OF CYTOTOXICITY AND GENOTOXICITY OF NEW SUBSTITUTED PIRYDO [1,2-A] BENZIMIDAZOLES USING THE ALLIUM TEST

Investigations were carried out into mutagenic and mitosis modifying action (Allium test) of imidazole new polycyclic condensed derivatives having a high ability to embed into DNA molecules: 7-trifluor methyl pyrido [1,2- a] benzimidazole, 7- nitropyrido[1,2-a] benzimidazole, 7- amino pyrido [1,2- a] benzimidazole, 8-nitro- 7-trifluor methyl pyrido[1,2-a]benzimidazole, 8-amino- 7-trifluor methyl pyrido[1,2-a] benzimidazole. The evaluation of cytotoxic effect of compounds under investigation was performed by comparing with toxicological characteristics of a widely used DNA –intercalator 9-amino acridine. All azagetero cycles induced reduction of the prophase index evidencing their impact on the process of DNA replication. The most wide spread mutations were “bridge” type pathologies as a result of reunion of different daughter cells chromosomes and obliteration and losses of entire chromosomes at cells division. All mono- and di-substituted pirido[1,2-a] benzimidazoles produced a lesser mitodepressive and mutagenic action than 9-amino acridine. Monosubstituted pirido[1,2-a]benzimidazoles had lesser cytotoxicity and mutagenicity in the A cepa test-system.

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