THE EXPRESSION AND POLYMORPHISM INFLUENCE OF GENES, ENCODING THE GABA-RECEPTORS, ON SEVERITY OF THE ETHANOL DEPRESSING ACTION IN RATS
Abstract
Expression and polymorphism of encoding the Gaba-receptors of genes, which determine the severity of the depressing effect of ethanol in white outbred rats were studied. In the course of the experiment, genotyping of animals was made using 16 single-nucleotide replacements in genes encoding the GABA. The genetic diversity of allelic variants was revealed, based on 11 polymorphisms. It was found out that in rats with the genotype homozygous variant TT (rs 197587817) the expression level of gene Gabra l (in brain 8 h after administration of ethanol) was authentically lower (2-fold) as compared to the heterozygous genotype CT. An authentic association of the gene Gabrb1 expression level (p<0.05) with the severity degree of the ethanol depressing effect on the nervous system in rats 8 h after administration of the toxicant was demonstrated.
About the Authors
Natalya Sergeevna Osechkina
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Russian Federation
Russian Federation
Georgiy Valerevich Nazarov
Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy
Russian Federation
Russian Federation
Evgeniy Yur’evich Bonitenko
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Russian Federation
Russian Federation
Maksim Borisovich Ivanov
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Russian Federation
Russian Federation
Vadim Anatol’evich Kashuro
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Russian Federation
Russian Federation
Nataliya Vadimovna Lapina
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Russian Federation
Russian Federation
Aleksandr Vladimirovich Babkin
Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy
Russian Federation
Russian Federation
Irina Sergeevna Berdinskih
Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy
Russian Federation
Russian Federation
Aleksandra Sergeevna Melekhova
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Russian Federation
Russian Federation
Karina Olegovna Voytsekhovich
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Russian Federation
Russian Federation
Dmitriy Sergeevich Lisitskiy
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Russian Federation
Russian Federation
Natal’ya Vasil’evna Makarova
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Russian Federation
Russian Federation
Tat’yana Vladimirovna Kashina
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Russian Federation
Russian Federation
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Review
For citations:
Osechkina N.S., Nazarov G.V., Bonitenko E.Yu., Ivanov M.B., Kashuro V.A., Lapina N.V., Babkin A.V., Berdinskih I.S., Melekhova A.S., Voytsekhovich K.O., Lisitskiy D.S., Makarova N.V., Kashina T.V. THE EXPRESSION AND POLYMORPHISM INFLUENCE OF GENES, ENCODING THE GABA-RECEPTORS, ON SEVERITY OF THE ETHANOL DEPRESSING ACTION IN RATS. Toxicological Review. 2014;(6):22-27. (In Russ.)
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